5.18.3. An another mechanism for decay of mRNA
Several time because of the strong secondary RNA structure formation within the open reading frame (ORF) the ribosomes stall on the mRNA. That means the ribosome is not able to move on the mRNA thus called as No-go decay.
The Dom34 and Hbs1 proteins bind the transcript near the stalled ribosome and initiate an endonucleolytic cleavage event near the stall site. This releases the ribosome and generates two mRNA fragments, each with a free end exposed for exonucleolytic decay by the exosome and Xrn1, respectively.