22.5. Nucleic acid stability
Many forces stabilizing the DNA structure
22.6. Thermal Stability
The temperature at which 50% DNA in given sample become denatured, known as melting temperature (Tm). The double stranded DNA show the less absorbance at 260 nm. While the denature DNA show the more absorbance at 260 nm this phenomena known as hyperchromatic effect.
Factors affecting Tm